Monitoring enzymatic ATP hydrolysis by EPR spectroscopy.
نویسندگان
چکیده
An adenosine triphosphate (ATP) analogue modified with two nitroxide radicals is developed and employed to study its enzymatic hydrolysis by electron paramagnetic resonance spectroscopy. For this application, we demonstrate that EPR holds the potential to complement fluorogenic substrate analogues in monitoring enzymatic activity.
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عنوان ژورنال:
- Chemical communications
دوره 50 55 شماره
صفحات -
تاریخ انتشار 2014